The active fractions from size-exclusion were loaded into a Mono S column or Sulfopropyl sepharose fast flow (SPFF) for further purification. higher affinities to DNA substrates. In a dimeric enzyme, the two active sites work in tandem, with the faster site functioning before the slower one, and in the monomeric … Continue reading The active fractions from size-exclusion were loaded into a Mono S column or Sulfopropyl sepharose fast flow (SPFF) for further purification
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